Cloning and expression of a hybrid transforming growth factor-beta-1/transforming growth factor-beta-2

摘要

The present invention relates to hybrid TGF-β1/TGF-β2 precursor proteins, nucleotide sequences encoding the hybrid TGF-β1/β2 precursors, and methods of producing transforming growth factor-β2 using the nucleotide sequences encoding the hybrid precursors. Embodiments of the invention include hybrid TGF-β1/β2 precursors comprising the amino acid sequence substantially as depicted in Fig. 1b from about amino acid residue number 1 to about amino acid residue number 390 or from about amino acid residue number 30 to about amino acid residue number 390. In another embodiment, a nucleotide sequence of the invention comprises the nucleotide coding sequence substantially as depicted in Fig. 1b from about nucleotide residue number -70 to about nucleotide residue number 1755.