EXPRESSION SYSTEM FOR THE SECRETION OF BIOACTIVE HUMAN GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR (GM - CSF)

摘要

A B S T R A C TA gene expression system is used to produceheterologous biologically active proteins, inparticular bioactive granulocyte, macrophage-colonystimulating factor ("GM-CSF"), secreted from a hostselected from the Streptomyces genera. The geneexpression system includes a regulatory nucleotidesequence linked to a second nucleotide sequenceencoding the heterologous protein. The regulatorysequence, encodes a peptide which directs thesecretion of the heterologous protein in bioactiveform from a host selected from the Streptomycesgenera. The regulatory sequence includes a signalsequence and a promoter sequence. The secondnucleotide sequence, which encodes GM-CSF or abiologically active derivative of GM-CSF, may beeither natural or synthetic. The product of thisinvention is disulphide-bonded, non-glycosylated,oxidized, therapeutically useful GM-CSF from a hostselected from the Streptomyces genera.