Large scale prodn. of vaccine against Japanese encephalitis in cultured cell lines - provides pure vaccine of low cellular DNA content, comprises harvesting virus-contg. supernatant from cell cultures, opt. several times from same cells

摘要

Large scale prodn. of vaccine against Japanese encephalitis comprises:(a) inoculating cultured cells of a cell line with Japanese encephalitis virus (JEV) in the presence of viral multiplication medium;(b) propagating and multiplying JEV in the cells;(c) recovering the medium which comprises a suspension of virus produced by the cells;(d) purifying the viral suspension by = 1 of ion-exchange chromatography and gel permeation, and(e) forming the virus suspension into a pharmaceutical compsn. to ensure its preservation until use. Also claimed is the vaccine produced as above which contains 100 pg/dose DNA.