HUMAN TUMOROUS NECROTIC FACTOR, PROCESS OF ITS PREPARATION, DNA ENCODING THE HUMAN TUMOROUS NECROTIC FACTOR, A CELL TRANSFORMED BY THIS DNA, AGENT IN WHICH THE HUMAN TUMOROUS NECROTIC FACTOR IS COMPRISED AND AGENT SUITABLE FOR TREATING TUMORS

摘要

Human tumor necrosis factor, which a) is not glycosylated, b) by SDS-PAGE has a molecular weight of 17,000, c) is able to preferentially destroy or inhibit tumor cell growth as compared to normal cells under the same conditions, d) is by SDS-PAGE is substantially homologous; and e) comprising: i) the amino acid sequence of residues 35-66 of formula I, iii) the amino acid sequence of residues 110-133 of formula I or iv) the amino acid sequence of Glu Thr Pro Glu Gly Ala Glu Ala Lys Pro Trp Tyr Glu Pro. A method for producing human tumor necrosis factor by isolation from a composition comprising human tumor necrosis factor and a heterogeneous mixture of contaminating proteins. DNA encoding human tumor necrosis factor, a cell transformed with this DNA, a composition comprising human tumor factor and a composition suitable for treating tumors.ŕ