New adenoviral vectors, packaging cell lines, recombinant adenoviruses and methods thereof (NOVEL ADENOVIRAL VECTORS, PACKAGING CELL LINES, RECOMBINANT ADENOVIRUSES AND METHODS)

摘要

The present invention provides novel adenoviral (E1) and non-adenoviral (E1) adenoviruses characterized in that at least two fatal initial gene portions of the gene portions E1 and E4 that normally transcribe adenoviral early proteins are deleted /RTI Such novel recombinant vectors are used in human gene therapy regimens wherein the vector further comprises a trim gene or therapeutic gene that replaces the E1 or E4 portion. The present invention provides novel packaged cell lines in mammals transgenic to the adenoviral E1 and E4 gene segments and to propagate adenoviral vectors without novel replication competence.