kondoroichinaze mutation body of I and kondoroichinaze II productivity P. burugarisu

摘要

(57) Abstract Extrinsic chondroitin when it breeds under absence of I and II inducer, P. burugarisu kondoroichinaze the mutation Proteus burugarisu stocks which produce I and the kondoroichinaze II protein are offered. As for this variant, typically, kondoroichinaze I and II protein, under absence of the extrinsic inducer, is produced at more excessive tonnage than those protein which is produced furthermore by the wild die P. burugarisu stocks which are induced with that kind of inducer. Commute two classes and class 1 of mutation body and 2 are disclosed. Class 1 and the class 2 mutant differs the cell kondoroichinaze in I which is produced to the case where it bred with the minimum nutrient medium which the kazamino Acid is acquired and II relative tonnage. The further phenotype mutation cell which separates, is similarly offered the kondoroichinaze I protein to in the culture medium. In addition P. burugarisu kondoroichinaze also the manner which produces I and II protein is intended. The aforementioned mutation cell, usual extrinsic kondoroichinaze is cultured under absence of I and II inducer, after that, the cell is harvested, and is recovered from the cell where kondoroichinaze I and II is harvested. In addition, kondoroichinaze manner of insaichiyu detecting I by the bacterial colony and II production is offered.