DIAGNOSTIC METHOD, KIT AND MANUFACTURING METHOD OF HERORRHAGIC FEVER WITH RENAL SYNDROME USING WESTERN BLOT

摘要

The present invention comprises Western blot the nucleocapsid protein, a specific antigen of nephrotic hemorrhagic fever-induced virus, on a nitrocellulose membrane, and reacting the sample and control serum with the nitrocellulose membrane onto which the antigen is blotted. Detecting the antigen-antibody reactant generated by using a biotin-labeled goat anti human immunoglobulin G antibody, streptavidin-labeled alkaline phosphatase and BCIP / NBT (BCIP / NBT), respectively, and a reaction sample. The present invention relates to a diagnosis method for nephrotic bleeding hemorrhagic fever comprising a step of comparing the detection result in the control serum and a diagnostic kit using the same.;According to the present invention, it is possible to diagnose nephrotic bleeding fever accurately and easily without a skilled inspector or special equipment.