RECOMBINANT PLASMID DNA PSP64-S PROVIDING IN VITRO TRANSCRIPTION RNA S-SEGMENT FRAGMENT OF VIRUS-PATHOGEN OF HEMORRHAGIC FEVER WITH RENAL SYNDROME OF SEROTYPE HANTAAN AND POSITIVE RNA CONTROL FOR DIAGNOSIS OF HEMORRHAGIC FEVER WITH RENAL SYNDROME BY METHOD RT/PCR BASED ON THEREOF

摘要

FIELD: biotechnology, genetic engineering, medicinal virology, biochemistry. SUBSTANCE: invention proposes recombinant plasmid DNA pSP64-S comprising promoter SP6 of DNA-dependent RNA-polymerase and cDNA S-segment fragment of virus-pathogen of hemorrhagic fever with renal syndrome (HFRS) of serotype Hantaan. Plasmid carries out the effective transcription of negative RNA chain from S-segment that is identical with virus genome RNA fragment. Also, invention proposes positive RNA control for diagnosis of HFRS virus-pathogen. For preparing the proposed positive RNA control plasmid pSP64-S is used as matrix. Proposed invention safety in carrying out works in preparing positive RNA control for diagnosis of HFRS virus-pathogen. EFFECT: simplified and enhanced safety, enhanced control quality. 2 cl, 1 dwg, 2 ex