The eukaryotic cell line is useful in the identification and Quantification of inhibitors of the processing Route of beta Amyloid Molecules of DNA, the design of this cell line.Methods of identification, High Throughput Assay and Monoclonal Antibodies, compositions

摘要

A eukaryotic cell line that has a building whose Construction exogenous Gene, exogenous Gene is a sequence codificantede a cytomegalovirus Promoter.A sequence encoding a ribosome binding site, a Coding sequence of - amyloid precursor protein B,A sequence encoding a selectable Marker and a sequence encoding a polyadenylation Signal.This line of cu00e9lulaseucarioticas are capable of producing more than 30 ng of Protein Molecule beta Amyloid 4,2kda per mg of Protein.For the design of the llu00edneascelulares uses a Nucleic Acid Molecule substantially Purified eukaryotic cell lines are useful in a method for Identifying the uninhibidorProcessing Route of beta Amyloid,To manage such a line: The inhibitor of eukaryotic cells and quantify Protein unamolu00e9cula indicative of inhibition of processing Route of beta Amyloid.They are useful in a method of identification of uninhibidor the processing Route of beta Amyloid.Consisting of (a) hatching inhibitor processing of beta Amyloid and celulareucariotica line to produce a Protein Molecule that is indicative of inhibition of Route prAnd Aereal Photographs: processing of beta Amyloid in a medium containing an amino acid Labeling and (b) separating the eukaryotic cell line and the Environment; (c) to separate the Protein Molecule of the cell line and aucariotica(d) environment and quantify the Protein Molecule.Another method is a high throughput assay for the identification of an inhibitor of processing of beta Amyloid,(a) incubarun inhibitor consisting in the processing of beta Amyloid and a eukaryotic cell line to produce a Protein Molecule