Micropropagation of shoots, rooted shoots, or seedlings, by complete immersion in oxygenated liquid medium

摘要

Shoots, rooted shoots, and seedlings of a woody plant are micropropagated by completely immersing them in an oxygenated liquid medium. The liquid medium can be oxygenated by passing oxygen or air through it. The woody plant can be a gymnosperm or dicotyledenous angiosperm used for wood pulp, fuel, or timber; a tree, shrub or bush that produces fruit or nuts; or an ornamental tree or shrub. This process is also useful for reducing microbial contamination of shoots, rooted shoots, or seedlings. A process for the selection of genetically modified plant material is described, where the genetically modified plant material is micropropagated according to the method described above.