mesothelial cells of visceral pleura

摘要

In a method for providing mesothelial cells or a mesothelzellinie from the visceral pleura of mammals, an insulated mammal lung in a mixture containing a protease, serum-free incubation buffer at approximately 37 deg c, the released cells isolated and with a serum-free culture medium, wherein the protease mixture preferably a mixture of collagenase type iv and dispase is.