Helicobacter and Wolinella species-specific detection and identification using new oligonucleotide primers directed to 16S rRNA consensus sequences

摘要

Detection and identification of Helicobacter and Wolinella species through use of oligonucleotide primers directed to 16S rRNA consensus sequences at positions corresponding to 540-584 nucleotides (nt) (sequence (I)) and 1173-1206 nt (sequence (II)) is new. All sequences are fully defined in the specification. A method for species-specific detection and species identification of bacteria of the species Helicobacter and Wolinella comprising treating a DNA or RNA sample to be investigated so that only a special gene fragment of the 16S rRNA is amplified, when the sample contains bacteria of the genus Helicobacter or Wolinella. At least one novel oligonucleotide primer of about 18 bp is used for the method, which are localized to consensus regions A and B of 16S rRNA corresponding to positions 540-584 nt (I) or 1173-1206 nt (II) of sequences fully defined in the specification. Amplification is carried out by using the chosen oligonucleotide primers fixed to the nucleotide sequence by a known molecular biology method, for example PCR. In the event of a positive detection, the resulting amplification product can be sequenced by a known DNA sequencing technique using the described oligonucleotide primers or internal primers as sequencing primers and identification of the Helicobacter species in question from the resulting nucleotide sequence by sequence comparison.