New primer opposite in order to amplify between the ITS field from the 16SrRNA gene or the 16SrRNA gene of potato scab cause germ genus, and the detection identification mannered null potato scab of the potato scab cause germ genus which

摘要

PROBLEM TO BE SOLVED: To provide a primer pair which is used for PCR and can strain-specifically amplify the genome DNAs of potato scab-causing strains, and to provide a method by which the specification of a strain in the speck tissues of scab-diseased tubers, the specification of the presence or absence of the scab strain and the strain in a potato field, and the specification of cultured scab fungi can quickly and simply be carried out at low costs.;SOLUTION: This primer pair for PCR hybridizes to a strain-specific base sequence of a 16SrRNA gene of the scab strain and a 16S-23S internal transcribed spacer (ITS), wherein DNA fragment sizes (base pairs) to be amplified are clearly different among the strains. A method for amplifying a genome DNA between hybridized primers, and a method for identifying an amplified product.;COPYRIGHT: (C)2006,JPO&NCIPI