A method for identifying Quantitative Trait Loci in a population of Plants defined combination, comprising a multiplicity of Families of PlantsA method for selecting plants with a desired phenotype, a Plant Well selected, a method for producing a transgenic Plant

摘要

It provides a method to identify quantitative trait loci (QTL) in a defined population of plants, combined, which comprises unamultiplicidad Families of PlantsThe Method comprises: 1) to quantify the phenotypic trait Lines taken as samples of the poblacioncombinada,Thus providing a phenotype of Quantified Population; (ii) identify at least one Genetic Marker associated with a phenotypic trait distribucionde,By tracking a group of markers for Associations with the phenotype of The Quantified Population; and (iii) identify the loci of cunatificativos traits on the basis of the AssociationThe phenotypic trait and a genetic Marker. In One embodiment, the population of Plants consists of diploid plants, either inbred or Hybrid.The phenotypic traits Preferred include but are not limited to; yield of grains, the Grain Moisture content, Oil content of the grains.The structures of the roots, the overturning of lostallos, Plant Height, STEM Height, disease Resistance and insect Resistance.In a way realizacionpreferida, Genetic Markers associated with QTLs are: the restriction fragment length polymorphism markers, isozymes,Lahidridacion specific alleles Amplified sequences, the variables of the plant genome sequence, the replication of self-sustainingSequencing simple repetitions and polymorphs of arbitrary length of the Fragment.In another embodiment is Preferred, at least two genu00e9ticosestu00e1n markers associated with QTLs and are identified by a Mass Screening.The Association of Genetic loci and phenotypic trait is determined through specific modelosestadu00edsticos.In a Preferred embodiment, the statistical models are linear models with fixed Effects, and Effects aelatorios.A particularly Preferred embodiment, the statistical model is a model with combined Effects, where the Progeny of a phenotypic trait