首页> 外国专利> Isolated Nucleic Acid fragment that encodes all or a substantial portion of an inhibitory Protein of apoptosis in plants and Seeds, Chimeric Gene, transformed host cell.Polypeptide, method for altering the expression level of this protein in a host Cell, Methods for

Isolated Nucleic Acid fragment that encodes all or a substantial portion of an inhibitory Protein of apoptosis in plants and Seeds, Chimeric Gene, transformed host cell.Polypeptide, method for altering the expression level of this protein in a host Cell, Methods for

机译:编码植物和种子中凋亡抑制蛋白的全部或大部分的分离的核酸片段,嵌合基因,转化的宿主细胞多肽,改变该蛋白在宿主细胞中表达水平的方法

摘要

Reveal isolated Fragments of nucleic acids which encode Proteins that inhibit apoptosis.Specifically,A single Fragment of u00e1cidosnucleicos that encodes a protein dadl or AAC - II and a fragment of nucleic acids which is complementary to the Fragment of nucleic acid and Protein codificaunaI or AAC - 11.It also reveals a polypeptide that encodes all or a substantial portion of the Protein and a Chimeric Gene comprising Nucleic Acid fragment which encodes the Protein or its complemEntario operably linked to regulatory sequences, where appropriate, the expresiondel Chimeric Gene results in the production of altered levels (i.e.Increased or decreased) of the encoded protein in a cu00e9lulahuu00e9sped transformed with respect to the levels produced by a non transformed host cell.The transformed host Cell, it is revealed, can be of eukaryotic or prokaryotic origin, and includes cu00e9lulasderivadas of Higher Plants and Microorganisms.It Additionally provides a methodof alter the expression level of a protein dadl or AAC - ll in a host Cell transformed and a method to obtain a fragment of nucleicoquecodific acidAll or a substantial portion of an amino acid sequence of the Protein Coding and the products obtained.
机译:显示编码抑制凋亡蛋白的核酸的分离片段。具体来说,是一个编码蛋白dadl或AAC-II的单核糖核酸片段以及与该核酸和蛋白codificaunaI或AAC片段互补的核酸片段-11,它还揭示了一种编码全部或大部分蛋白质的多肽,以及一个包含核酸片段的嵌合基因,该核酸片段编码该蛋白质或其组成部分,可操作地连接至调控序列的Entario,在适当的情况下,expresiondel嵌合基因导致产生相对于非转化宿主细胞产生的水平,转化的编码蛋白的变化水平(即增加或减少)。揭示了​​该转化宿主细胞可能是真核或原核的,并包括高等植物和微生物的c.ulusderivadas。此外,它还提供了一种更改表达式的方法转化宿主细胞中的蛋白dadl或AAC-11的水平和获得核酸quedcodific acid的片段的方法和所得蛋白的全部或大部分氨基酸序列和产物。

著录项

  • 公开/公告号AR014731A1

    专利类型

  • 公开/公告日2001-03-28

    原文格式PDF

  • 申请/专利权人 E.I. DU PONT DE NEMOURS AND COMPANY;

    申请/专利号AR1999P101141

  • 发明设计人

    申请日1999-03-16

  • 分类号C12Q1/68;

  • 国家 AR

  • 入库时间 2022-08-22 01:26:23

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