Isolation and purification of an inhibitor of piroglutamil Aminopeptidase II from anu00c9lido Hermodice carunculata Marine and Product thus obtained

摘要

It establishes for the first time a procedure of isolation and purification of a natural inhibitor of piroglutamil amonopeptidasa II from extracts of Marine Hermodice carunculata have read.The Protocol consists of three basic Stages: Treatment of the extract with Trichloroacetic acid at 2.5% of the anion exchange Chromatography on DEAE - and Sephacel molecular Exclusion Chromatography on Sephadex g-25.The homogeneity of the obtained fraction (80% higher yields and degree of purification, High)Was tested by obtaining a narrow and symmetrical Single Peak on rp-hplc and obtaining a single polyacrylamide gel electrophoretic Band in sds-page.