Method for sexing of sperm using the classical pathway of complement system, with elimination of the alternative route by inactivation of the protein 'B

摘要

Method for sexing of sperm using the classical pathway of complement system, with elimination of the alternative route by inactivation of the protein "B".The production method for the imunossexagem sperm cattle using monoclonal antibodies associated with proteins of the classical pathway complement system is the goal of thisThe patent. Monoclonal antibodies directed against the proteins associated with the sex specific causes cell lysis by complement proteins via the classical complement system.For this methodology can be used, it is necessary to the elimination of the alternative route of complement, which is the basis of our technology, where we eliminate the action of protein B,Responsible for the activation of the alternative route, by heating the serum of guinea pigs to 52n 0 C sym 0,3 per 30 minutes.The sperm are placed in contact with the specific monoclonal antibody against the protein marker of sexual characteristic.Then, add saline is heated so as to only via the classical complement system is activated to promote the specific lysis of spermatozoa marked.Thus, the sperm in the semen treaty will be ready to be processed according to the purpose, insemination, cryopreservation or in vitro.