Method for providing an increased level of security for controlling transgenic segregation in a sexually reproducing multicellular organism and / or for providing automatic prevention of transgene leakage, DNA construct complex, cloning vector cells or cell lines of a sexually multicellular organism. (srmo), transgenic plant, and transgenic nonhuman sexually reproductive multicellular organism

摘要

"METHOD FOR PROVIDING AN INCREASED SAFETY LEVEL TO CONTROL TRANSGENIC SEPARATION IN A SEXUALLY REPRODUCTIVE MULTI-CELL ORGANISM AND / OR TO PROVIDE AUTOMATIC TRANSGENE CULTURE COMPLEX OR CELL CONSTRUCTION COMPLEX LEAKAGE SEXUALLY REPRODUCER (SRMO), TRANSGENIC PLANT, AND SEXUALLY REPRODUCTIVE MULTI-CELL ORGANISM ". The invention relates to a method and complex of DNA constructs for providing an increased level of transgene segregation control in Sexually Reproductive Multicellular Organisms (SRMOs), which are prone to crossbreeding with their wild type or cultured relatives. The method and constructs allow the farmer to reuse the GM crop without risk. The RBF system comprises, in addition to one or more Transgenes of Interest (TGIs) encoding the desired genetic products, one or more Blocking Constructs (BC) and one or more user controlled means for retrieving locked functions. The BC has the ability to block at least one essential function for survival and / or reproduction of the SRMO. Preferably, more than one BC flanking TGIs are used. The BC may be located in close proximity to the TGI, preferably in an intron or flanking the TGIs. The locked function is recoverable by user controlled interventions, preferably applicable under confined conditions. The intervention is combined with one or more Recovery Constructs (CRs). Different types of RBF systems are described. The RBF Double, Triple and Segregant systems and the intron inserted BC are preferred embodiments of the present invention. The Transgenic Inserts (TI) of the Triple RBF system and their interactions are shown in Figure (10).