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PRIMERS FOR AMPLIFYING HSP 65 GENE OF MYCOBACTERIAL SPECIES, HSP 65 GENE FRAGMENTS AND METHOD OF IDENTIFYING MYCOBACTERIAL SPECIES WITH THE SAME
PRIMERS FOR AMPLIFYING HSP 65 GENE OF MYCOBACTERIAL SPECIES, HSP 65 GENE FRAGMENTS AND METHOD OF IDENTIFYING MYCOBACTERIAL SPECIES WITH THE SAME
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机译:用于扩增菌种HSP 65基因,HSP 65基因片段的鉴定方法以及用相同方法鉴定菌种的方法
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摘要
The present invention relates to a pair of primers specific to mycobacterial species, a polynucleotide of an hsp 65 gene fragment, and a method for the identification of mycobacterial species by using the same. More specifically, the 604-bp hsp 65 gene fragment can be applied to identification methods of mycobacteria such as the comparative sequence analysis method, the probe hybridization method, and PCR-RFLP, which can resolve the problems of a conventional identification method based on biochemical characteristics, where the genus mycobacterium covers various species and has a low growth rate, and of the problems of 16s rDNA. Thus, according to the identification method of the present invention, the mycobacterial species can be identified simply, economically, and accurately.
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