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Primers for amplifying hsp 65 gene of mycobacterial species, hsp 65 gene fragments and method of identifying mycobacterial species with the same
Primers for amplifying hsp 65 gene of mycobacterial species, hsp 65 gene fragments and method of identifying mycobacterial species with the same
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机译:用于扩增分枝杆菌种的hsp 65基因的引物,hsp 65基因片段以及用其鉴定分枝杆菌种的方法
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摘要
The present invention relates to a pair of primers specific to mycobacterial spedes, a polynucleotide of an hsp 65 gene fragment, and a method for the identification of mycobacterial species by using the same. More specifically, the 604-bp hsp 65 gene fragment can be applied to identification methods of mycobacteria such as the comparative sequence analysis method, the probe hybridization method, and PCR-RFLP, which can resolve the problems of a conventional identification method based on bio-chemical characteristics, where the genus mycobacterium covers various species and has a low growth rate, and of the problems of 16s rDNA. Thus, according to the identification method of the present invention, the mycobacterial species can be identified simply, economically, and accurately.
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