Separation and Production Methods of Caffeine Epigallocatechin Epicatechin Epigallocatechingallate and Epicatechingallate from Tea Leaves

摘要

PURPOSE: Separation and production methods of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate from tea leaves are provided, thereby simultaneously separating and producing the five kinds of materials. CONSTITUTION: Separation and production methods of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate from tea leaves comprises the steps of: steaming tea leaves and drying them; heat-extracting the dried tea leaves or extracting the extract thereof with EtOH and freeze-drying it; subjecting the powdery extract to the sephadex column to obtain fractions of caffeine, epigallocatechin, epicatechin, epigallocatechingallate, and epicatechingallate; and subjecting the fractions to HPLC, removing an organic solvent and freeze-drying the HPLC fractions.