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Gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene
Gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene
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机译:编码调节水稻防御相关基因表达的转录因子的基因和利用该基因诱导系统获得性抗性的方法
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摘要
PURPOSE: Provided are gene encoding for a transcription factor which regulates the expression of defense related genes in rice and method for inducing the systemic acquired resistance by using this gene. In particular, provided is Oryza sativa ethylene response element binding protein(OsEREBP), a transcription factor which regulates the expression of defense related genes in rice. CONSTITUTION: The method of selecting OsEREBP comprises: treating the known systemic acquired resistance inducer, benzo-1,2,3-thiadiazole-7-carbothioic acid-S-methyl ether (BTH) to produce mRNA which is related to induction of systemic acquired resistance; transcribing the mRNA to obtain cDNA clone by using a transcriptase; selecting cDNA clone capable of being amplified by BTH treatment using the cDNA as a probe; and confirming a OsEREBP by analyzing nucleotide sequence and homology of cDNA clone. The nucleotide sequence of OsEREBP is represented by SEQ ID NO:1.
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机译:目的:提供编码调节水稻中防御相关基因表达的转录因子的基因,以及利用该基因诱导系统获得性抗性的方法。特别地,提供了稻米乙烯反应元件结合蛋白(OsEREBP),其是调节水稻中防御相关基因表达的转录因子。组成:选择OsEREBP的方法包括:处理已知的全身性获得性耐药诱导剂苯并1,2,3-噻二唑-7-硫代甲酸-S-甲基醚(BTH)以产生与诱导全身性获得性耐药相关的mRNA抵抗性;通过转录酶转录mRNA以获得cDNA克隆;以所述cDNA为探针,选择能够通过BTH处理扩增的cDNA克隆。通过分析cDNA克隆的核苷酸序列和同源性来确定OsEREBP。 OsEREBP的核苷酸序列由SEQ ID NO:1表示。
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