Procedure for the rapid Synthesis of a Peptide in Solution methods for the synthesis of Combinatorial Libraries of peptides and to the automated Synthesis of peptides in solution that apply this procedure and a Peptide or a Peptide mixture thus obtained (a)

摘要

A procedure for the rapid Synthesis of a Peptide in Solution, which involves repeated Cycles of the steps (a) - (d): (a) a phase coupling using an excess of one component to acilar amino carboxylic activated Component, (b) a phase of extinction that uses an agent Sweeper for removing functions carboxu00edlicas activated SewageDone Agent Sweeper can also be used for the Protection of the Peptide in Growth.(c) one or more aqueous Extracts and Optionally, (d) a separate deprotection step, followed by one or more aqueous extractions, where the procedure includes at least one Stage (stage b), called (B '), in which an Amine or thiol which comprises an anion anion free or Latent Sweeper is used as an agent of the functions carboxu00edlicas activated sewage.The anion present in the Amine Sweeper has a temporary Protective Group, Benzyl type, More specifically a protected amino acid derivative in the C - terminal, such as the Beta Alanine. The Sweeper is a Beta alaninate Agent benzyl thiol anion, comprising a Polyamine or a free or Latent.The Procedure includes extraction is carried out in the presence of Sodium Chloride or Potassium nitrate. During the procedure the Peptide on Growth need not be isolated until it has obtained the final sequence of the peptide. This procedure is useful for highly efficient production of oligo - and polypeptides of high purity.Also disclosed are methods for the synthesis of Combinatorial Libraries of peptides and to the automated Synthesis of peptides in solution to apply the procedure and a Peptide or a Peptide mixture thus obtained (a).