AN IMPROVED PROCESS FOR SIMULTANEOUS PREPARATION OF SEX SPECIFIC AND GENDER NEUTRAL SEMISYNTHETIC AMPLICONS USEFUL FOR SEX DETERMINATION

摘要

An improved process of preparation of sex specific and gender neutral semisynthetic amplicons useful for sex determination, which comprises, isolating nucleic acids from any part of a papaya plant by conventional methods, amplifying the said nucleic acids in a conventional Random amplification of polymorphic DNA Polymerase Chain Reaction (RAPD-PCR), resolving the amplified products by conventional electrophoresis mthod, eluting the sex specific, double stranded, amplified product form the gel piece by known methods, synthesizing the single stranded chanins of synthetic oligonucleotides from the said double stranded amplified product by known method, amplifying the said single stranded chain of synthetic oligonucleotide in a conventional Polymerase Chain Reaction by using synthetic oligonucleotides as primers to get desired sex specific and gender —neutral semisynthetic amplicons.