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METHOD FOR DETECTING PHYTOESTROGEN FROM CHINESE MEDICINE BY USING GAS CHROMATOGRAPHY-MASS SPECTROMETER
METHOD FOR DETECTING PHYTOESTROGEN FROM CHINESE MEDICINE BY USING GAS CHROMATOGRAPHY-MASS SPECTROMETER
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机译:气相色谱-质谱联用法检测中草药中植物雌激素的方法
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摘要
PURPOSE: Provided is a method for detecting phytoestrogen from chinese medicine by using gas chromatography-mass spectrometer, which can eliminate a pretreatment step of ion exchange resin used for removing an inhibition material of analysis. CONSTITUTION: The method comprises the steps of (i) removing impurities from object to be detected by using solid phase extraction, so as to obtain a phytoestrogen, and (ii) deriving the obtained phytoestrogen by using TMS(trimethylsilylation), wherein the derivation step(ii) comprises converting ketone group into hydroxyl group in the phytoestrogen. In the step(ii), TMS is performed by using a derivation reagent comprising MSTFA(N-methyl-N-trifluorotrimethylsilyl acetamide), NH4I and dithioerythritol.
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