Primer for detection of Newcastle disease virus, and methods for detection, pathotyping and genotyping of the Newcastle disease virus using the same

摘要

PURPOSE: Provided are primers for detection of newcastle disease virus, and methods for diagnosing the newcastle disease by simultaneously performing detection and pathotyping thereof and for genotyping of the newcastle disease virus by RFLP(restriction fragment length polymorphism). CONSTITUTION: The primers for detection of newcastle disease virus are characteristically represented by the SEQ ID NO:1 and 2. The primers for pathotyping of the newcastle disease virus are characteristically represented by the SEQ ID NO:3 and 4. The method for genotyping of the newcastle disease virus is characterized by RFLP using Hinf I and Pst I as restriction enzymes, and PCR products using the primers of SEQ ID NO:1 and 2.