PRIMER AND KIT FOR DETECTION AND PATHOTYPE-DIAGNOSIS OF NEWCASTLE DISEASE VIRUS AT THE SAME TIME, THEREBY REDUCING DEATH RATE OF DOMESTIC ANIMALS BY THE NEWCASTLE DISEASE VIRUS

摘要

PURPOSE: A primer and kit for detection and pathotype-diagnosis of Newcastle disease virus are provided, thereby simultaneously detecting the Newcastle disease virus and diagnosing pathotype of the Newcastle disease virus, so that death rate of domestic animals by the Newcastle disease virus can be reduced. CONSTITUTION: The primer for detection and pathotype-diagnosis of Newcastle disease virus is selected from a primer set having the nucleotide sequences of SEQ ID NO:1 and SEQ ID NO:2, a primer set having the nucleotide sequences of SEQ ID NO:2 and SEQ ID NO:3, and a primer set having the nucleotide sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3. The method for pathotype-diagnosis of Newcastle disease virus comprises the steps of: (1) obtaining RNA from a sample; (2) carrying out reverse transcription of the RNA to obtain cDNA; (3) PCR amplifying the cDNA using the primer; (4) subjecting the PCR product to electrophoresis; and (5) deciding the Newcastle disease virus as non-pathogenic virus when the PCR product shows a band of 379bp, or as pathogenic virus when the PCR product shows two bands of 379bp and 204bp.