Extract Method for Resveratrol and Its Derivatives isolated from Paeonia lactiflora Seeds and Its Use for Improved Bone Diseases

摘要

PURPOSE: A method for extracting resvedratrol and derivatives thereof as four stilbene compounds from a Paeoniae radix seed is provided. Whereby, the compound can be used for a substitute of 17β-estradiol, genistein promoting the formation of a bone and having estrogen activity. CONSTITUTION: This resvedratrol and derivatives thereof are extracted by the process consisting of: removing grease from dried Paeoniae radix seed powder by adding n-hexane; obtaining a methanol extract by adding methanol to defatted meal and repeatedly extracting; adding methanol to the extract and absorbing in an HP-20 column; fractionally eluting with methanol; concentrating two ultraviolet absorption fractions(I, II, III) under reduced pressure; and separating trans-resvedratrol-4-o-β-D-glucoside and trans-resvedratrol from fraction I and II and trans-viniferin and cis-viniferin from fraction III.