A method for the production of double-stranded nucleic acids with single-stranded overhangs of any desired length and nucleotide composition

摘要

A method for the production of deoxyribonucleic acid (dna) with one or two 5 ''- projecting ends characterized in that, on a dna - female die a dna - synthesis with one or two polymer excimer, ducks oligonucleotides by means of a dna - polymerase is carried out, with excimer is a polymer where oligonucleotide at least one nucleotide analogue at any desired position, which is not normally occurs in the dna and the nucleotide analog the following requirements are fulfilled:a) is a free or an already in a dna - strand built-in polymer excimer is where oligonucleotide as a matrix of a dna - new synthesis by means of a dna polymerase, -, there is a 5 ''- overhang, which begins with the nucleotide analogue and extending to the 5'' - end of the polymer is ducks oligonucleotide excimer extends.b) the nucleotide analogues of the polymer excimer, ducks oligonucleotide is in the layer, with deoxyribonucleic acid to enter into a nucleotide specific base pairing.c) the nucleotide analogues of the polymer excimer, ducks oligonucleotide is in the layer, with ribonucleic acid to enter into a nucleotide specific base pairing.d) the nucleotide analogues of the polymer excimer, ducks oligonucleotide does not impair the property of the polymer excimer, ducks oligonucleotide as a starter molecule - de novo synthesis of a dna to be able to serve...