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A method for the parallel isolation of double- and single-stranded nucleic acids and for the selective removal of double-stranded nucleic acids from a mixture of double-stranded and single-stranded nucleic acids
A method for the parallel isolation of double- and single-stranded nucleic acids and for the selective removal of double-stranded nucleic acids from a mixture of double-stranded and single-stranded nucleic acids
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机译:一种双链和单链核酸的平行分离方法,以及从双链和单链核酸的混合物中选择性去除双链核酸的方法
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摘要
The invention relates to a method for the efficient and extremely simple and inexpensive parallel isolation of double-stranded and single-stranded nucleic acids. According to the invention, the double-stranded nucleic acid in the absence of alcohol or alkaline earth ion complexing substances or washing, dispensing or wetting agents with an aqueous salt solution in a concentration greater than 1 M is adjusted so that the double-stranded nucleic acid is adsorbed to a solid support, while the single-stranded Nucleic acid is not adsorbed and remains in the solution.
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