STABLE INTEGRATION OF DNA IN BACTERIAL GENOME.

摘要

A bacterial cell which in its genone carries an integrated non-replicative DNA construct comprising (1) a DNA sequence of interest, (2) a DNA sequence which is homologous with a region of the genome of the cell, and (3) an origin of replication, said DNA construct lacking a functional gene coding for a factor required to initiate replication from said origin of replication.