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LIGASE / POLYMERASE-MEDIATED ANALYSIS OF GENETIC ELEMENTS OF SINGLE-NUCLEOTIDE POLYMORPHISMS AND THEIR USE IN GENETIC ANALYSIS
LIGASE / POLYMERASE-MEDIATED ANALYSIS OF GENETIC ELEMENTS OF SINGLE-NUCLEOTIDE POLYMORPHISMS AND THEIR USE IN GENETIC ANALYSIS
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机译:连接酶/聚合酶介导的单核苷酸多态性遗传元素分析及其在遗传分析中的应用
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摘要
A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.
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