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PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method
PCR amplification method, PCR primer set, PCR amplification product, and method for detection of nucleic acid using the amplification method
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机译:PCR扩增方法,PCR引物组,PCR扩增产物以及使用该扩增方法的核酸检测方法
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摘要
A nucleic acid amplification method capable of preferentially amplifying a nucleic acid strand having a desired nucleotide sequence from a template nucleic acid molecule using a PCR amplification method, where the two primers flanking a base sequence region to be amplified are each designed so that they have different template-primer melting temperature (Tm) under PCR conditions.
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