PCR amplification method, pcr primer set, pcr amplification product, and method for detection of nucleic acid using the amplification method

摘要

A nucleic acid amplification method capable of preferentially amplifying a nucleic acid strand having a desired nucleotide sequence from a template nucleic acid molecule using a PCR amplification method, where the two primers flanking a base sequence region to be amplified are each designed so that they have different template-primer melting temperature (Tm) under PCR conditions.