RECOMBINANT PLASMID DNA PFASTBAC-G2R COMPRISING SMALLPOX VIRUS GENOME FRAGMENT ENCODING PROTEIN-ANALOG OF TUMOR NECROSIS FACTOR RECEPTOR AND STRAIN OF BACULOVIRUS BTRI67 PRODUCING SOLUBLE RECEPTOR OF SMALLPOX VIRUS TUMOR NECROSIS FACTOR

摘要

FIELD: genetic engineering, molecular biology, biotechnology.;SUBSTANCE: method involves using polymerase chain reaction (PCR) method for isolating gene G2R encoding the protein-analog of human tumor necrosis factor-alpha receptor from the smallpox virus genome of the strain "India-1967". Then this gene is cloned in donor plasmid pFastBac and the recombinant bacmid is reconstructed by site-specific transposition method. Method involves transfection of insect cells with recombinant bacmide for generation of recombinant baculovirus BTRi67 expressing gene G2R in the insect cell line Sf21. Invention provides the high level of synthesis of the end product and its correct post-translation modification. Invention can be used for preparing a medicinal agent of the new generation used for control of human severe diseases associated with hyperproduction of tumor necrosis factor-alpha (TNF-alpha).;EFFECT: valuable biological and medicinal properties of plasmid and strain.;3 cl, 3 dwg, 6 ex