Method to diagnose the severity of a disease in a indivu00ecduo test, and test kit for screening a sample of patient for the presence of circulating cancer cells

摘要

"Method to diagnose the severity of a disease in a indivu00ecduo test, and.Test kit for screening a sample of patient for the presence of circulating cancer cells, "a highly sensitive test is presented.Which uses a method for magnification prepared specific genes, collections of rare cell mRNA and transcripts rarely found in blood.The test enables the detection of rare mRNA (10 copies / cell) found in 1 to 10 cells isolated by enrichment imunomagnu00e9tico.The test is an improvement on the PCR multicomplexa and allows the detection of sequences coding for efficient rare carcinomas cells circulating in the blood.The methods are useful in profiling of cells isolated from tissues or body fluids and serve as an adjunct to clinical diagnosis of multiple carcinomas.Including the detection of early stage and classification of tumor cells circulating in the blood.The monitoring of nucleic acid and protein profiles of cells, or in conventional formats or microarranjos, facilitates the control of therapeutic intervention, including staging,The response of the monitoring to the therapy, the confirmation of remission and the detection of the regression.