A method for the production of transgenic plants which express a protein, which is a hydrogen - peroxide prod ornamental end activity, by means of transformation by agrobacterium rhizogenes

摘要

The invention concerns a method for obtaining transgenic plants expressing a protein activity producing H2O2 comprising the following steps which consist in: (a) transforming plant cells with Agrobacterium rhizogenes containing a vector bearing a gene coding for a protein producing H2O2 in a context which enables its expression in the plant; (b) selecting transformants which contain and express said gene by a peroxydase colorimetric test; (c) regenerating the plants from selected roots and controlling the expression of the resulting plantlets by a peroxydase colorimetric test; (d) phenotypic screening and optionally molecular analysis of the transgenic plants progeny, enabling to select and confirm the resulting transgenic plants containing only the transgene and not the T-DNA belonging to the Agrobacterium rhizogenes. The invention is useful for obtaining transgenic plants.