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Preparing hapten from doxycycline and bovine serum albumin, used to raise antibodies for detecting the antimalarial doxycycline in serum or urine
Preparing hapten from doxycycline and bovine serum albumin, used to raise antibodies for detecting the antimalarial doxycycline in serum or urine
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机译:用强力霉素和牛血清白蛋白制备半抗原,用于制备抗体来检测血清或尿液中的抗疟药强力霉素
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摘要
Method for preparing a hapten (I) by Mannich reaction between doxycycline (II) and bovine serum albumen (BSA) comprises (a) dissolving (II) and BSA in presence of formaldehyde at pH 4-5; (b) incubation of the mixture at 25-50[deg]C for at least 12 hours and (c) purification and isolation of the (II)-BSA conjugate formed by passage through a desalting column. ACTIVITY : Antimalarial. MECHANISM OF ACTION : None given.
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