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Immunochromatography of psilocin class by competitive method detection method and test kit

机译:竞争法检测方法和检测试剂盒对psilocin类进行免疫层析

摘要

PROBLEM TO BE SOLVED: To provide an immunochromatography detection method for specifically detecting psilocin by a simple method with high sensitivity.;SOLUTION: A membrane carrier (3) is prepared and has a first catching part (31), formed by fixing a substance having the antigenic determinant of the psilocin to a specified position, in advance. Chromatographic development is performed on the mixed liquor of an antibody (I) to the psilocin and a sample to be tested in the membrane carrier toward the first catching part, and the psilocin contained in the sample to be tested is detected by competitive reaction to the antibody (I) of the substance immobilized to the first catching part and the psilocin contained in the sample to be tested. Preferably, the membrane carrier is equipped with a second catching part (32) formed, by immobilizing an antibody (II) to the antibody (I) at the downstream side of the chromatographic development at the first catching part.;COPYRIGHT: (C)2005,JPO&NCIPI
机译:解决的问题:提供一种免疫色谱检测方法,以简单,高灵敏度的方法特异性检测psilocin。解决方案:制备膜载体(3),并具有第一捕获部分(31),该捕获部分通过固定含有预先将psilocin的抗原决定簇定位到指定位置。在膜载体中朝着第一个捕获部分的抗loclocin的抗体(I)和待测样品的混合液中进行色谱显影,并通过与psilocin的竞争反应来检测待测样品中所含的psilocin。固定在第一捕获部分的物质的抗体(I)和待测样品中所含的psilocin。优选地,膜载体装备有第二捕获部分(32),该第二捕获部分通过将抗体(II)固定在色谱展开的下游侧的抗体(I)上的第一捕获部分处。版权所有:(C) 2005,日本特许厅

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