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Messenger RNA Profiling: Body Fluid Identification Using Multiplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
Messenger RNA Profiling: Body Fluid Identification Using Multiplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
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机译:Messenger RNA分析:使用多重逆转录聚合酶链反应(RT-PCR)进行体液鉴定
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摘要
This invention relates to a body fluids identification method and kit. A parallel, multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay for the definitive identification of body fluids commonly encountered in forensic casework analysis, namely blood, saliva, semen, and vaginal secretions. The methodology is based on gene expression profiling analysis in which the body fluid-specific genes are identified by detecting the presence of appropriate messenger RNA species. Gene-specific primers are labeled with fluorescent dyes, separated and subjected to laser induced fluorescence for identification of body fluid-specific genes present in a sample stain.
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