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Producing an overexpressive strain of Staphylococcus aureus for producing a polysaccharide capsular, comprises culturing the strain on a culture medium and optionally recovering the produced strain from the medium
Producing an overexpressive strain of Staphylococcus aureus for producing a polysaccharide capsular, comprises culturing the strain on a culture medium and optionally recovering the produced strain from the medium
Producing an overexpressive strain of Staphylococcus aureus comprises pre-culturing the strain of Staphylococcus aureus on a culture medium comprising: wheat peptone (30 g); yeast extract (5 g); glucose (1 g); ferric chloride (120 mg); magnesium chloride (400 mg); calcium chloride (10 mg); sodium chloride (58.5 g); zinc chloride (5 mg); diammonium sulfate (6.8 g); ammonium chloride (6 g); and the pH value is 6.5, where the Staphylococcus aureus is of two serotypes (T5) and (T8). Independent claims are included for: (1) production of a polysaccharide capsular; (2) a medium adapted to the culturing of Staphylococcus aureus; (3) a culture medium M1 adapted to the polysaccharide capsular of the strain Staphylococcus aureus; and (4) an overexpressive strain of Staphylococcus aureus obtained by the new process. ACTIVITY : Antibacterial. MECHANISM OF ACTION : Vaccine.
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