Corrective method for processing results of transcriptomic experiments carried out on a DNA chip obtained by the differential analysis, comprises obtaining the results of the level of gene expression in a reference and treatment condition

摘要

The corrective method for processing the results of transcriptomic experiments carried out on a DNA chip obtained by the differential analysis, comprises obtaining the results of the level of gene expression in a reference and treatment condition, calculating the mean level of the expression of each genes, calculating a modulation coefficient of the expression level for each genes, calculating p-value associated with each modulation coefficient, and calculating isobar curve of the p-value in function of the expression level. The corrective method for processing the results of transcriptomic experiments carried out on a DNA chip obtained by the differential analysis, comprises obtaining the results of the level of gene expression in a reference and treatment condition, calculating the mean level of the expression of each genes, calculating a modulation coefficient of the expression level for each genes, calculating p-value associated with each modulation coefficient, and calculating isobar curve of the p-value in function of the expression level. Median modulation coefficient is calculated and associated over the observed isobar curve using global error assessment method. The calculations are carried out in different processing conditions. The calculation of p-value isobar curves includes a representation of a graph by a point of each gene in which logarithm of the mean level of the expression under the condition of reference is noted in x-axis and logarithm of the mean level of the expression under the condition of treatment is noted in y-axis. The isobar curve corresponds to theoretical points for which p-value is equal to p. A user selects the genes on the basis of a value of modulation coefficient and a value having a materiality. An independent claim is included for a computer for implementing the corrective method.