Indoxyl sulfate derivative, antigen, antibody and method for detecting indoxyl sulfate using the same

摘要

PROBLEM TO BE SOLVED: To obtain a new derivative capable of rapidly, accurately and simply carrying out diagnosis and examination of uremia by determining a specific indoxyl sulfate by immunological determination. ;SOLUTION: This derivative is represented by formula I {L¹ to L³ are each a group of formula II [X is O, S, N, etc.; Y¹ and Y² are each an aromatic ring containing a 5-membered or 6-membered heterocycle, a single bond, etc.; (n), (m) and (p) are each 0-5]}, e.g. sodium 1-(2',3'epoxypropyl)indoxyl sulfate. Furthermore, the compound of formula I is preferably obtained by using a compound of formula III (p¹ is OH group-protecting group; p² is an amino group-protecting group; X¹ is a halogen) as a starting substance, dissolving the starting substance in an organic solvent such as dimethyl formamide and reacting the compound of formula III with L²X² (X² is a halogen, etc.) at -78C to ambient temperature, as necessary, together with a catalyst such as metal sodium.;COPYRIGHT: (C)1998,JPO