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Multiplex PCR Assay For Identification of USA300 and USA400 Community-Associated Methicillin Resistant Staphylococcal Aureus Strains

机译：多重PCR鉴定USA300和USA400社区相关的耐甲氧西林金黄色葡萄球菌菌株

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摘要

The present invention relates to multiplex polymerase chain reaction (PCR) assays for Staphylococcus aureus typing. In particular, the invention relates to identification, detection and classification of USA300 and USA400 strains, the predominant community-associated methicillin-resistant S. aureus (MRSA) strains in North America, as well as other strains, and simultaneous detection of PVL genes and discrimination of MRSA from methicillin-susceptible S. aureus (MSSA) and S. aureus from coagulase-negative staphylococci (CoNS).

机译：本发明涉及用于金黄色葡萄球菌分型的多重聚合酶链反应（PCR）测定法。特别地，本发明涉及USA300和USA400菌株的鉴定，检测和分类，USA300和USA400菌株是主要的社区相关的耐甲氧西林的I。北美的金黄色葡萄球菌（MRSA）菌株以及其他菌株，并同时检测PVL基因和从易感甲氧西林的 S鉴别MRSA。金黄色（MSSA）和 S。凝固酶阴性葡萄球菌（CoNS）产生金黄色葡萄球菌。 展开▼

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公开/公告号US2009111134A1

专利类型

公开/公告日2009-04-30

原文格式PDF

申请/专利权人 KUNYAN ZHANG;JOHN CONLY;
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申请/专利号US20080061847

发明设计人 KUNYAN ZHANG;JOHN CONLY;
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申请日2008-04-03

分类号C12Q1/48;

国家 US

入库时间 2022-08-21 19:33:44

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机译：用于鉴定USA300和USA400社区相关性耐甲氧西林金黄色葡萄球菌金黄色葡萄球菌菌株的多重PCR检测

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9. Novel Multiplex PCR Assay for Simultaneous Identification of Community-Associated Methicillin-Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton-Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci▿ [O] . Zhang, Kunyan, McClure, Jo-Ann, Elsayed, Sameer, 2007

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