STRAIN OF MYCELIAL MUSHROOM PENICILLIUM VERRUCULOSUM - PRODUCER OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE AND WAY OF RECEPTION OF FERMENTAL PREPARATION OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE FOR HYDROLYSIS OF CELLULOSE AND HEMICELLULOSE

摘要

FIELD: medicine; microbiology.;SUBSTANCE: fermental preparation of a complex cellulase, xylanase and xyloglucanase for hydrolysis of cellulose and hemicellulose is obtained by propagation of the Penicillium verruculosum VKM F-3972D strain on the water medium containing in g/l: cellulose - 50-60, glucose - 10-30, mineral salts - KH₂PO₄ 8-12, (NH₂)₂SO₄ 3-7, MgSO₄·7H₂O 0.2-0.4, CaCl₂·2H₂O 0.2-0.4, at continuous feeding with glucose. The propagation process is conducted at temperature of 26-30°C and pH in a range of 4.5-5.0. The culture liquid containing a fermental preparation, is separated in 120-144 h after propagation beginning, treated with ultrafiltration and dried up.;EFFECT: high specific activity of the complex of cellulase, xylanase and xyloglucanase and rising of efficiency of use of a fermental preparation in various areas of biotechnology.;2 cl, 1 tbl, 3 ex