METHOD AND DEVICE FOR NANOPORE BASED SINGLE-MOLECULE PROTEIN/ PROTEIN INTERACTION DETECTION

摘要

The present invention relates to a method and a device for nanopore based single-molecule protein/protein interaction detection. The invention combines the use of a charged polymer to drive a target molecule to the entrance of a nanopore, for the charged polymer to thread through the pore, and the molecule attached to the polymer to be held at the entrance of the nanopore as a dumbbell. Trans-membrane ionic current measurements can be used to monitor the ion conductance of a single nanopore. Dissolved analytes can be detected if they enter and interact with the nanopore inner channel by altering its conductance. Depending on the pore dimensions, single molecules can be detected in this way.