METHOD FOR ANALYZING CIRCULATING FREE DNA IN PLASMA AND A METHOD FOR DIAGNOSING OR DETECTING DISEASE RELATING TO CPG ISLAND METHYLATION USING THE SAME

摘要

PURPOSE: A method for analyzing circulating free DNA in plasma is provided to effectively diagnose or detect disease relating to CpG island methylation.;CONSTITUTION: A method for analyzing circulating free DNA in plasma comprises: a step of performing primary PCR with a primary PCR primer pair in order for unmethylated cytosine to contains plural CpG sites to circulating free DNA converted to thymine or uracil; a step of performing secondary nested PCR with secondary nested PCR primer pair; and a step of performing SSCP analysis to the PCR product. The circulating DNA in plasma is tumor-relating gene. A method for diagnosing or detecting diseases relating to CpG island methylation comprises: a step of analyzing circulating free DNA in plasma; and a step of diagnosing or detecting the diseases from the result.;COPYRIGHT KIPO 2010