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X-ray coordinates defining the human papillomavirus E2 transactivation domain / inhibitor co-crystal and the inhibitor binding pocket
X-ray coordinates defining the human papillomavirus E2 transactivation domain / inhibitor co-crystal and the inhibitor binding pocket
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机译:定义人乳头瘤病毒E2反式激活域/抑制剂共晶体和抑制剂结合口袋的X射线坐标
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摘要
A crystallizable composition, comprising an PV E2 TAD-like polypeptide of SEQ ID NO.2 complexed with an inhibitor L. The invention also provides a method for producing the crystallized HPV E2 TAD-inhibitor complex (HPV E2 TAD-L) comprising: a) mixing purified HPV E2 TAD, contained in a purification buffer, with solublized inhibitor L to generate a complex solution containing the HPV E2 TAD-L complex; and b) crystallizing the complex from a) in a crystallization buffer. The invention also provides a method for producing crystallized apo HPV E2 TAD, comprising: a) mixing apo HPV E2 TAD, contained in a purification buffer, with a crystallization buffer. X-ray crystal structure coordinates the HPV E2 TAD-L complex, are also provided, which define an inhibitor binding pocket. The inhibitor binding pocket is useful for screening potential small molecule inhibitors that bind to the pocket.
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