METHOD FOR CREATING A VIRAL GENOMIC LIBRARY, A VIRAL GENOMIC LIBRARY AND A KIT FOR CREATING THE SAME

摘要

A method for creating an alphavirus-based genomic library, comprising a) ligation of foreign sequence (s) from an expression library or a random library into plasmids containing cloned alphaviral cDNA, b) multiplication of the obtained plasmid constructs in bacterial cells, c) direct transfection of the obtained plasmid constructs into mammalian or arthropod cells, characterized in that the sequence of an intron or sequences of introns are inserted into the respective genome of an alphavirus or into the cDNA of an expression vector based on an alphavirus, —the sequence of a viral subgenomic promoter, which is larger than minimal functional promoter is inserted immediately to the 3′ end of the sequences coding the structural proteins of the named alphavirus, —and ribozyme sequence is inserted for creating correct 3′ ends of the alphavirus.