METHOD FOR IN VITRO CULTURE OF BOVINE CLONED FERTILIZED EGGS FOR 20 HOURS BY ADDING 50nM TRICHOSTATIN A INTO A CULTURE MEDIUM

摘要

PURPOSE: A method for in vitro culture of bovine cloned fertilized eggs is provided to enhance cell generation rate.;CONSTITUTION: A method for in vitro culture of bovine cloned fertilized eggs comprises: a step of preparing a culture medium in stock form and storing in a refrigerator or freezer; a step of treating 50nM of trichostatin A to the basic culture medium under the presence of 0.4% BSA(bovine serum albumin); a step of culturing the bovine cloned fertilized eggs in vitro; a step of isolating cells from bovine ear and establishing somatic cell line; a step of removing cumulus cells using hyaluronidase for enucleation; a step of pricking the zona pellucida using a enucleation pipette and inhaling cytoplasm and first polar body; a step of transplanting a donor cell to the enucleated egg; a step of injecting one donor cell; and a step of fusing and activating.;COPYRIGHT KIPO 2011